The Reds take on Julian Nagelsmann’s side at the Rhein-Neckar-Arena on Tuesday night, before the second leg at Anfield on August 23.
Representing one of the most important clashes in recent years for Liverpool, Klopp will be desperate to secure progress to the Champions League group stages at Hoffenheim’s expense.
Youngsters Conor Masterson and Cameron Brannagan are surprise inclusions, but are likely named to fill the Reds’ quota for locally trained players.
However, given the two legs are to be held before the end of the transfer window, this does not serve as definitive proof of his plans for the upcoming campaign.
Liverpool Squad for Champions League Play-Offs
Goalkeepers: Mignolet, Karius, Ward
Defenders: Matip, Lovren, Klavan, Gomez, Masterson*, Clyne, Alexander-Arnold*, Moreno, Robertson, Flanagan
Midfielders: Coutinho, Henderson, Wijnaldum, Can, Milner, Grujic, Brannagan*, Ejaria*, Woodburn*
Forwards: Firmino, Sturridge, Origi, Solanke, Mane, Salah, Kent*, Ojo*, Wilson*, Brewster*
* Locally trained player.
All 20 clubs involved in the upcoming play-offs were required to submit their two squad lists to UEFA by midnight on Monday, with a maximum of 25 players on their List A.
Two places are reserved for goalkeepers, while a minimum of eight places are reserved for locally trained players (either by club or association).
List B can include an unlimited number of players born on or after January 1, 1996 who have been eligible to play for the club for at least two years since their 15th birthday.
Therefore, Klopp has named a total of 32 players for the two-legged play-off, and, if required, can add one further player by midnight on the day before the clash.