The Reds head back to Anfield after departing for World Cup qualifiers and friendlies, taking on familiar opposition in Mauricio Pellegrino’s Saints.
Liverpool have signed five players from Southampton in the past three years, while Pellegrino spent two-and-a-half years on Merseyside, first as a player and then as assistant manager.
Klopp was unsuccessful in his efforts to sign Virgil van Dijk last summer, leaving the Dutchman to line up against his suitors on Saturday.
Adam Lallana, who has returned from a long-term thigh injury, is not part of the squad.
Liverpool are currently on a three-game winning streak, and with 13 games over the next 45 days, including 11 outings in the league, another victory is essential on Saturday.
Liverpool: Mignolet; Alexander-Arnold, Lovren, Klavan, Moreno; Henderson, Wijnaldum, Coutinho; Salah, Mane, Firmino
Substitutes: Karius, Gomez, Milner, Can, Oxlade-Chamberlain, Sturridge, Solanke
Southampton: Forster; Cedric, Van Dijk, Hoedt, Bertrand; Romeu, Davis; Tadic, Boufal, Redmond; Long
Substitutes: McCarthy, Yoshida, Targett, Hojbjerg, Ward-Prowse, Gabbiadini, Austin