The Reds need at least a point from their final game of Group E to ensure their place in the last 16, with a win guaranteeing a first-placed finish.
They take on Spartak on the back of an eight-game unbeaten run, and back-to-back victories over Stoke City and Brighton, with the latter a convincing 5-1 away rout.
But the pressure will be on at Anfield, and Klopp has named a strong starting lineup in his bid to take the three points.
His selection for this all-important encounter underlines this, as Liverpool head out under the lights for their most important game of the season so far.
Liverpool: Karius; Gomez, Lovren, Klavan, Moreno; Can, Wijnaldum, Coutinho; Salah, Mane, Firmino
Substitutes: Mignolet, Alexander-Arnold, Henderson, Milner, Oxlade-Chamberlain, Solanke, Sturridge
Spartak Moscow: Selikhov; Eshchenko, Tasci, Bocchetti, Dzhikiya; Fernando, Glushakov; Zobnin, Adriano, Promes; Ze Luis
Substitutes: Rebrov, Kutepov, Samedov, Melgarejo, Pasalic, Popov, Pedro Rocha