Kostas Tsimikas was not part of Liverpool’s training session in front of the cameras on Tuesday, with two players now doubtful for the clash with Villarreal.
In the hours leading up to training, the manager revealed that it would be a “surprise” if Roberto Firmino was available, and the Brazilian subsequently missed the team workout.
However, Klopp also outlined his hope that no new injuries had emerged in the days since the Merseyside derby.
It remains to be seen whether the left-back is injured, but Tsimikas was not involved as the squad warmed up for the open section of training on the eve of the game.
That would not necessarily rule him out against Villarreal, but it would suggest that he has at least been advised to work separately if there has been a fitness concern.
The midfield pair have endured a torrid run of injuries throughout their careers at Liverpool, but are emerging as key players for the run-in this time around.
Liverpool squad in training on Tuesday
Defenders: Van Dijk, Matip, Konate, Gomez, Alexander-Arnold, Robertson
Forwards: Salah, Mane, Diaz, Jota, Origi, Minamino