They do so having secured top spot in their group, with the eight clubs who finish second instead advancing to a playoff round.
Fixtures across Tuesday and Wednesday confirmed that Galatasaray, Lens, SC Braga, Benfica, Feyenoord, AC Milan, Young Boys and Shakhtar Donetsk will drop down from the top-tier tournament.
The winners of those playoffs will be drawn against a group winner in the last 16, with the latter also playing the second leg at home.
As it stands, Sporting CP are the only side already guaranteed second in their Europa League group, with Liverpool one of only three confirmed winners along with Atalanta and Bayer Leverkusen.
Sevilla, who have won the Europa League five times in the last 10 years including last season, also failed to progress.
Union Berlin, Red Bull Salzburg, Celtic, Red Star Belgrade and Royal Antwerp were the other sides knocked out.
The knockout playoffs for the Europa League will be held on February 15 and 22, with the draw for the last 16 held on February 23.
After that, the last 16 will take place on March 7 and 14, meaning after their trip to Union SG, Liverpool will not play again in Europe for almost three months.